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Preliminary phytochemical screening and antitrichomonal activity of Ferula pseudalliacea
Zarifeh Akbari1 , Dara Dastan2 , Mohammad Fallah1 , Amir Hossein Maghsood1 , Mohammad Matini3
1- Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
2- Department of Pharmacognosy, School of Pharmacy, Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
3- Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran , matini@umsha.ac.ir
Abstract:   (304 Views)
Background and objectives: Trichomonas vaginalis causes human trichomoniasis, a common type of protozoan vaginitis. Due to the increasing incidence of drug-resistant trichomoniasis, new pharmacological research is needed. The aim was investigation of the activity of Ferula pseudalliacea against T. vaginalis, and preliminary phytochemical analysis of its extracts.
Methods: Essential oil and various extracts of F. pseudalliacea roots, including n-hexane, ethyl acetate and methanol, were obtained. Susceptibility testing of the plant products was done on five T. vaginalis isolates by using microtiter plate method. Minimum lethal concentration (MLC) and growth inhibitory percent (GI%) of sub-MLC concentration were reported, after 24 and 48 hours’ exposures. Phytochemical screening of the extracts was done using standard procedure.
Results: Antitrichomonal effect of the plant products depended on time and concentration. The greatest effect was observed in 48-hour exposure. The essential oils and n-hexane extract of F. pseudalliacea demonstrated a remarkable activity with MLC of 250 µg/ml and following by the ethyl acetate (MLC=500 µg/ml) and methanol extract (MLC=1000 µg/ml), with GI% 92.8, 50.6, 85.2, and 42.8, respectively. The bioactive constituents of the extracts were coumarins, terpenoids, steroids, phenols, tannins and glycosides.
Conclusion: The results of this study demonstrated in vitro antitrichomonal properties of F. pseudalliacea. Therefore, further studies are needed to investigate the potential of the antitrichomonal activity of its bioactive constituents. 
Keywords: Antitrichomonal agents, Essential oil, Extract, Ferula pseudalliacea, Phytochemicals, Trichomonas vaginalis
Full-Text [PDF 475 kb]   (92 Downloads)    
Research Article: Research Article | Subject: Parasitology
Received: 2024/01/7 | Accepted: 2024/04/20
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