This paper should be cited as Khaki, A.

Effect of Ciprofloxacin on Sexual Hormones and Spermatocytes in Rat

Khaki, A. (PhD)

Associate Professor of Pathology, Faculty of Veterinary, Islamic Azad University, Tabriz, Iran

Abstract

Background and Objectives:Ciprofloxacin is a synthetic antibacterial agent belonging to Fluoroquinolone drugs affecting effectively on gram-negative bacterial infectious.The aim of this study was to assess the effect of ciprofloxacin in the spermatogenesis period.

Material and Methods:The subjects were 20 male wistar rat randomly divided in to control (n=10) and experimental group (n=10), given 12.5mg/kg ciprofloxacin (soluble in drinking water) in spermatogenesis period. On the day of 28,the sperm was collected from cauda epididymis and sent for analysis.

Results:Based on light microscopic observation and statistical analysis, the majority of seminiferous tubules of control group were healthy, in Sc 8-9 stage.But in test group, sertoli cell degeneration and absence of sex cells were confirmed,and in some parts, just basal layer of seminiferous tubule was remained in Sc 3-5 stage.

Conclusion:Sex hormones (LH and FSH) and spermatogenesis (sperm count, motility and viability) were significantly decreased in test group compared to those of controls (P<0.05). Ciprofloxacin has some adverse effects on sperm related variables in 28 day period.

Keywords:Ciprofloxacin,Sperm,Sex Hormones

Corresponding Author: Khaki, A.

Email: arashkhaki@iaut.ac.ir

Received 19 Jul 2013 Revised 20 Sep 2013 Accepted 23 Sep 2013