Mohit Kumar, Jayaprakash C S, Athira K P,
Volume 19, Issue 1 (4-2025)
Abstract
Background and objectives: Non-Hodgkin lymphoma represents a heterogenous group of lympho-proliferative malignancies with unique presentation and treatment response. This study was intended to assess the histomorphology of non-Hodgkin lymphoma subtypes and correlate with clinicopathological and immunohistochemical findings.
Methods: A retrospective study was conducted on all samples, diagnosed as non-Hodgkin lymphoma by histopathological analysis, with available immunohistochemical findings. Study was conducted in the department of pathology, for 24 months, during the period of March 2018 to February 2020. The specimens were fixed in 10 % formalin and representative sections were obtained.
Clinical details such as age, gender, location and presentation were recorded. Histopathological and immunohistochemical findings were correlated. Statistical analysis based on p value was performed.
Results: The study includes a total of 48 cases with a male predominance (56.3%). Cervical lymph node (35.4%) was the most common site. Extra nodal presentation was noted in 39.6% of the cases including stomach, testis, hard palate, para-sacral area and para-vertebral area. Diffuse large B-cell lymphoma (43.8%), followed by follicular lymphoma and mantle cell lymphoma were the most common subtypes. A statistically significant p value of 0.05 was obtained.
Conclusion: In our study, we found diffuse large B cell lymphoma (DLBCL) as the most common type of Non Hodgkin’s Lymphoma. Cervical lymph nodes were most common site of involvement. But involvement of sites like the testis and palate were also found, hence the probability of NHL in these rare sites should always to be considered.
Jithu Jacob, Swapna C Senan, Ramani Bhai,
Volume 19, Issue 1 (4-2025)
Abstract
Aim: The global distribution of Klebsiella pneumoniae that produce carbapenemase has been gradually increasing.This present study aimed to investigate the molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates from various clinical samples. Materials and Methods: In this study, 401 bacteria of Klebsiella isolates were isolated from various clinical samples according to standard protocol. The twelve carbapenem-resistant genes of Klebsiella pneumoniae isolates were detected using multiplex polymerase chain reaction (PCR). Results: Multiplex polymerase chain reaction (PCR) for identifying Class A β-lactamases producers (KPC), Class B β-lactamases producers (NDM), and Class D β-lactamases producers (OXA-48) were done. It was noted that 10 isolates expressed KPC followed by one isolate expressed NDM and one isolate expressed OXA-48. Conclusion: In the present study conclude that CP-CRK is a major health problem in the coming years and hence it is necessary to take all adequate measures to identify the resistant strains. Continuous monitoring of these resistant mechanisms is required to establish the changes in the prevalence and sensitivity pattern of MDR Klebsiella isolates. Urgent infection control measures coupled with antibiotic stewardship and strengthening of the healthcare infrastructure are to be instituted in our setting to prevent the spread of these Carbapenem-resistant genes of Klebsiella pneumoniae (KPC). Larger multicenter studies are required to thoroughly assess risk variables and historical trends in order to comprehend the dynamics of spread and efficient management strategies.
