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Showing 3 results for Faraji
Comparison of Molecular (PCR) and Pap Smear Methods in Diagnosis of Human Papiloma Virus (HPV) in Women with Genital Warts
H Bashi Zadeh Fakhar, R Faraji, M Ghane, M Jafarpour, B Ashoorizadeh,
Volume 7, Issue 3 (Autumn 2013)
Abstract
Abstract Background and Objective: Much research has shown that Human Papiloma Virus (HPV) plays an important role in cervix cancer and it is the cause of 99% of cervix cancer worldwide. Lots of research has been done to find a proper method for HPV diagnosis and screening in patients with genital warts. This study aimed at comparing PCR method with Pap smear test in HPV screening. Material and Methods: Considering the presence of DNA of HPV, 45 vaginal and cervix swap samples of women with genital warts were tested by means of specific PCR and Pap smear from September 2010 to April 2011. Results: Out of 45 vaginal and cervix swap samples of women suffering genital warts, 37 samples (82.2%) are positive. Of 45 Pap smear samples, 13 (29%) are neoplasia and 32 (71%) normal. Conclusion: The difference between the results of PCR and Pap smear is due to low specification and sensitivity of Pap smear. Thus it is recommended using diagnostic PCR method in addition to Pap smear in order to promote the quality of screening in individuals with genital warts. Keywords: Human Papiloma Virus (HPV) Genital Warts Molecular (PCR) Pap Smear
Prevalence and Antibiotic Resistance Pattern of Gram-Positive Isolates from Burn Patients in Velayat Burn Center in Rasht, North of Iran
Mobina Hosseini, Meysam Hasannejad-Bibalan, Tofigh Yaghoubi, Mohammadreza Mobayen, Parisa Khoshdoz, Sara Khoshdoz, Niloofar Faraji, Hadi Sedigh Ebrahim-Saraie,
Volume 15, Issue 6 (Special issue (Nov-Dec) 2021)
Abstract
Background and objectives: Bacterial contamination of wounds is a serious problem, particularly in burn patients. Gram-positive bacteria are the predominant cause of infection in newly hospitalized burn cases. This study aimed to survey the prevalence and antibiotic resistance pattern of gram-positive bacterial isolates among burn patients in Rasht, North of Iran.
Methods: This cross-sectional study was conducted on burn patients with a positive culture for gram-positive isolates who were hospitalized in the Velayat Burn Center in Rasht, North of Iran, during 2017-2020. The isolates were identified using standard microbiological methods. Moreover, the antibiotic resistance pattern was determined by the disk diffusion method.
Results: During the study period, 671 bacterial cultures were obtained, of which a total of 16 gram-positive isolates were taken from the patients. The frequency of coagulase-negative staphylococci (CoNS), Staphylococcus aureus, and Enterococcus spp. was 68.7%, 18.8%, and 12.5%, respectively. In addition, the highest rate of resistance in CoNS isolates was against trimethoprim/sulfamethoxazole. The highest rate of resistant among S. aureus isolates was recorded against penicillin. Moreover, Enterococcus faecalis isolates showed a high level of resistance to ampicillin, erythromycin, tetracycline, gentamicin, and ciprofloxacin. All isolates were susceptible to teicoplanin. Moreover, the frequency of methicillin-resistant S. aureus isolates was 66.7%.
Conclusion: Given the increasing prevalence of drug-resistant strains, especially in susceptible burn patients, it is imperative to analyze the bacterial etiology of nosocomial infections periodically and epidemiologically.
Antibiotic resistance genes in helicobacter pylori isolates from gastric biopsies of patients with gastrointestinal diseases in Golestan province, Iran
Azam Shareh , Behnoush Khasheii , Tayebeh Faraji , Masoud Khoshnia , Shaghayegh Anvari , Ailar Jamalli ,
Volume 19, Issue 4 (Jul-Aug 2025)
Abstract
Background: Antibiotic resistance in Helicobacter pylori infections can lead to treatment failure. This study aims to evaluate the antibiotic resistance to metronidazole, clarithromycin, and fluoroquinolone in H. pylori strains isolated from gastric biopsy specimens.
Methods: This study was conducted between 2016 and 2017 on 80 biopsy specimens obtained from Golestan province. Resistance to metronidazole (rdxA) and fluoroquinolones (gyrA) was determined using PCR. Mutations in the loci of the 23S rRNA gene associated with clarithromycin resistance were analyzed using PCR-RFLP with the BsaI and BbsI enzymes.
Results: In this study, 25% of H. pylori strains showed resistance to clarithromycin. Mutations in the A2143G locus (65%) and the A2142G locus (35%) were detected in these strains. Resistance to fluoroquinolones (27.5%) was observed, with the most common mutations being at the 91 amino acid position of aspartate (63.63%) and the 87 amino acid position of asparagine (36.36%). Resistance to metronidazole was not observed in any of the strains of this study, and concomitant resistance to clarithromycin and fluoroquinolones was observed in 13.75% of H. pylori strains.
Conclusion: According to our study, in Iran, the resistance of H. pylori to clarithromycin is increasing, which may lead to treatment failure. The mechanism of clarithromycin resistance is related to mutations in the A2143G and A2142G positions, and a mutation in the gyrA gene causes resistance to fluoroquinolones, which often occurs in the 91 amino acid position.
 

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