Showing 6 results for Parsa
S Abedian, A.m Mirabi, Mr Parsaee,
Volume 2, Issue 2 (Autumn – Winter 2009[PERSIAN] 2008)
Abstract
Abstract Background and objectives: Dendritic Cells are the most important of antigen presenting cells with an effective role in immune tolerance. This study, aims to clarify the role of IFN- β in induction on dendritic cells derived by monocyte in diabetes type1 to evaluate the T cells response to beta cell specific antigenic molecule. Material and Methods: In this research, peripheral blood mononuclear cells were isolated by phiCole and then dendritic cells generated from blood monocytes ,in Seven days, by adding granulocyte-monocyte colony stimulating factor and interleukin-4 with or without IFN-beta. MRNA was extracted by dendritic cells and cDNA was produced by reverse transcriptase enzyme. Then, Specific polymerase chain reaction for HLA-G was performed. In addition, Tcell proliferation with a mixed Leukocytic reaction evaluated between dendritic cell and T by means of MTT. Results: based on the results, IFN-β induces HLA-G molecule on dendritic cells. In addition, T cell proliferation responses in mixed leukocyte culture show significance difference between Case and control p<0.05. T cell proliferation was inhibited in their co-culture system affected by IFN-β Conclusion: In this study, we show that dendritic cells-treated IFN-β with expression of HLA-G molecule inhibited T cell proliferation and so, our results suggest that some of the IFN- β regulatory effects with expression of HLA-G can probably prevent from beta cell destruction. Key words: dendritic cells, Interferon Beta, Human Leukocytic Antigen-G.
Biranvand E, Abedian Kenary S, Ghaheri A, Rezaee M S, Hasannia H, Nasrolahi M, Parsaee Mr, Ahanjan M, Biranvand B, Ahmadi Basiri E,
Volume 5, Issue 1 (spring-summer[PERSIAN] 2011)
Abstract
Abstract Background and objectives: Interferon-Gamma and interferon Gamma receptor (IFNγ ⁄ IFNγR1) are the main genes associated with susceptibility to tuberculosis. We aimed at studying on interferon-Gamma Gene polymorphism(- 56 C/T) in people suffered from tuberculosis (TB). Material and Methods: In this case-control study, the subjects were 62 individuals with TB and 74 healthy ones. Genomic DNA was extracted by DNA isolation kit(Roche Corporation), and genotype identification was performed by polymerase chain reaction-Restriction fragment length polymorphism (PCR-RFLP). Chi square and logistic regression, using SPSS soft ware (version 18), was used to compare genotype and alleles between case and control groups. Results: The frequency of TT genotype in tuberculosis patients and healthy person are 43.5% and 17.5%, respectively. Based on Logistic regression (odd ration 0.148, p=0.0006), there is significant difference between Case and Control. In addition, the frequency of T allele is, in case group, 62.09 % the difference between case and control is significant, based on Logistic regression (odd ratio: 0.418, P=0.028). Conclusion: It is implied that -56C/T is associated with IFNγR1 promoter in tuberculosis patient. It is found to be associated with increased susceptibility to tuberculosis. Key words: Tuberculosis, IFNγR, PCR-RFLP
Hosseini, Sm, Farhang Ara, E, Yousefi Mashouf, R, Parsavash, S,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract
Abstract Background and Objective: The prevalence of different bacterial species and antibiotic resistance varies according to geographical conditions. Hence, we aimed to identify the prevalence of bacterial agents isolated from the urine culture and also investigate the antibiotic susceptibility of bacteria. Material and Methods: This cross-sectional study conducted on 7200 urine samples to identify the bacteria causing infections, using differential tests. The antimicrobial susceptibility was performed via disk diffusion method according to the Clinical and Laboratory Standards Institute (CLSI). Results: The most common bacteria were Escherichia coli (736 61.1%) and Klebsiella pneumonia (128 10.6%) and Enterococcus faecalis, (88 7.3%). The highest antibiotic susceptibility was related to Ciprofloxacin (174 14.45%) and the lowest to Amoxicillin (795 62.02%). Conclusion: Owing to the lowest resistance, it is recommended that Ciprofloxacin be used as the first line of treatment. In addition, Amoxicillin and Ampicillin should be used the least during primary treatment of urinary tract infections. Keywords: Antibiogram, Bacterial Resistance, Urine Culture
Moshtaghi, H, Parsa, M,
Volume 9, Issue 1 (March, April[PERSIAN] 2015)
Abstract
Abstract
Background and Objective: Automated Teller Machine (ATMs) is likely to be contaminated with various microorganisms specially pathogen germs. This may be due to their exposure to dust and their vast dermal contact with multiple users. This study investigated the bacterial contamination on the keyboard of ATMs and drug resistance of the bacteria isolated from them.
Material and Methods: the keyboards of 50 ATMs in Shahrekord city, Iran, were examined from October 2012 to February 2013. The sterile swab sticks moistened with Triptose soy broth were used for sampling. The bacteriological tests used were culture, biochemical test and agar disk diffusion method for antibiogram.
Results: All the samples were found to be contaminated with Coagulase negative staphylococci (57.54%), Bacillus species (21.92%), Staphylococcus aureus (19.18%) and coliform bacteria (1.36%). The resistance of Staphylococcus aureus was 92.8% to penicillin, 85.7% to amoxicilin، 71.4% to ampicillin, 57.1% to nytrofuran, 50% to tetracycline, 42.8% to erythromycin, 42.8% to gentamycin, 14.2 % to ciprofloxacin, 7.1% to trimethoprim and sulfamtuksazul. All species were susceptible to, ofloxacine, chloramphenicol, clindamycin, tobramycin, vancomycin and cefotaxime.
Conclusion: given the presence of pathogens on ATMs and their role in transferring the contamination, we recommend considering personal hygiene and periodically disinfecting the keyboards to reduce contamination.
Keywords: ATMs, Bacterial Contamination, Antibiogram
Moradi, M, Matini, M, Mohemmi, N, Maghsoud, A, Zahirnia, A, Parsa, F, Fallah, M,
Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)
Abstract
Background and Objective: Assessment of alimentary tract helminthes of rodents has a great zoonotic importance. This study aimed at determining the prevalence of helminth infections in rodents in Hamadan.
Material and Methods: a total of 100 rodents trapped from different parts of city were transported to laboratory. After anesthetizing by chloroform, the animals were undergone an operation to isolates the helminthes. The isolates were stained by Carmine and identified at the genus and species levels. Furthermore, age, sex, weight of rodent and size of various organs of body were determined.
Results: Totally, 62% of the rodents were infected to intestinal helminthes. All trapped rodents were Rattus norvegicus. Six species of helminthes, including three Nematode (45%), 3 Cestode (51%) and no Trematode were isolated from rodents. The infection rate for different helminthes was as follow: Hymenolepis nana 21%, H. diminuta 29%, Heterakis spomosa 43%, Strongyloides sp. 1% Trichuris muris 1% and Cysticercus fasciolaris 1%.
Conclusion: in this area, infection rate of alimentary tract helminthes in the Rattus norvegicus, especially zoonotic helminthes, is relatively high, and the rate of Cestodes is higher than those of Nematodes and Trematodes.
Key words: Prevalence, Helminthes, Alimentary tract, Rodents
Mina Parsa , Malahat Ahmadi , Habib Dastmalchi , Aliasghar Tehrani ,
Volume 11, Issue 6 (Nov - Dec 2017)
Abstract
ABSTRACT
Background and Objectives: Nowadays, the prevalence of multidrug-resistant pathogens such as
Pseudomonas aeruginosa is increasing worldwide. Many studies have been seeking new treatment strategies to treat infections caused by these microorganisms. Silver nanoparticles (AgNPs) along with L-arginine have significant antimicrobial effects and could be used as alternatives for ineffective drugs.
Methods: In this study, the antibacterial activity of AgNPs, L-arginine and various concentrations of AgNPs along with L-arginine (12.5 and 25 mg/ml) were investigated against
P. aeruginosa PAO1 using the broth macrodilution method.
Results: Minimum inhibitory concentration of AgNPs, L-arginine and AgNPs combined with 25 and 12.5 mg/ml L-arginine was 15.6 μg/ml, 25 mg/ml, 1.9 μg/ml and 3.9 μg/ml, respectively. Minimum bactericidal concentration of AgNPs, L-arginine and AgNPs combined with 25 and 12.5 mg/ml L-arginine was 31.2 μg/ml, 50 mg/ml, 3.9 μg/ml and 7.8 μg/ml, respectively.
Conclusion: Our study suggests that AgNPs along with L-arginine can be used as an alternative antibacterial agent against
P. aeruginosa, and might be useful for treatment of wound infections.
Keywords: Nanoparticles, Arginine,
Anti-Bacterial Agents,
Pseudomonas aeruginosa.
