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Resistance Pattern of Enterococci Isolated from Nosocomial Infections in the Hospitals Located in Gonbad and Gorgan Cities
Naghipoor, E, Raefi, A, Nasrollahiomran, A,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract

Abstract Background and Objective: Enterococci are normal flora of human body and considered as the third leading cause of nosocomial infections. The aim of this study was to determine drug resistance of Enterococcus species through biochemical methods. Material and Methods: One hundred twenty-eight of enterococcus suspected samples were isolated from gorgan and gonbad’s hospitals from April to June, 2013. The samples were cultured on blood agar, chrome-agar, EMB agar and some special cultures of isolation of Enterococcus species. Suspension of bacteria was grown in Mueller Hinton agar and the inhibition zone diameter was determined by disk antibiogram. Results: Of 128 samples, 109(85.15%) were enterococci faecalis and 19 (14.85%) Enterococcus Faecium. In all of 128 cases, eight showed resistance to amoxicillin, ten to ampicillin, five to gentamicin, five to ciprofloxacin, six to chloramphenicol, four to cephalexin and one to vancomycin. Conclusion: It seems to be necessary to use drug sensitivity test for having appropriate treatment and preventing from resistance strains. Keywords: Enterococci, Antibiotic Resistance, Antibiogram


Prevalence of Malassezia species isolated from skin of patients with seborrheic dermatitis reffered to Tonekabon clinics by PCR- Sequencing method
A Raefi, N Nasrollahi Omran, A Nazemi,
Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)
Abstract

Abstract

Background and Objective: Malassezia yeast is considered lipophilic normal flora of human skin and warm-blooded vertebrates. This fungus is an opportunistic pathogen in causing seborrheic dermatitis. In this study, the yeasts isolated from the crust of the patients with seborrheic dermatitis were identified by PCR-Sequencing.

Material and Methods: In this study, 65 samples of the skin of ear, nose and dandruff were cultured in selective medium Sabouraud agar and modified Dixon agar to prevent dehydration. After biochemical tests, ITS1-4 Universal PCR primers were used to determine the species of yeast.  Obtained PCR products were sequenced for the determination and identification of Malassezia species.

Results: Of nine samples obtained from scalp, four were Malassezia globosa, two Malassezia restricta, two Cryptococcus albidus and one Cryptococcus albidus milis.

Conclusion: The results of Malassezia globosa and Malassezia Restericta are very similar with those in studies elsewhere.

Keywords: Malassezia, Sequencing, Seborrheic Dermatitis, Tonekabon


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