Zahra Ahmadnia , Samane Rouhi, Hamed Mehdinezhad , Siamak Sabaghi , Alireza Firouzjahi , Mohammad Ranaei , Hossein Ghorbani , Maryam Pourtaghi, Mana Baziboron ,
Volume 18, Issue 4 (7-2024)
Abstract
Pasteurella species are one of the most common pathogenic bacteria in domestic animals, and they are seen more in people with weak immune systems. This research aims to investigate a case of a patient with multiple sclerosis from whose sputum Pasteurella multocida (P. multocida) was isolated. The patient was a 28-year-old man with multiple sclerosis who had persistent coughs due to food being stuck in his throat. The patient was a 28-year-old man with multiple sclerosis who had persistent coughs due to food being stuck in his throat. The primary diagnosis was pneumonia hydropneumothorax and complete collapse of the left lung. The patient's sputum culture after the first visit to the hospital was positive for P. multocida, which was not found in a second culture. In the subsequent cultures of the patient, Acinetobacter, Klebsiella, Pseudomonas aeruginosa (P. aeruginosa), and Citrobacter were found, which had extensive drug resistance to all antibiotics. In the secondary computerized tomography (CT) scan, mild pleural effusion on the left side, pneumothorax, and complete collapse with bronchiectasis was seen. Despite the treatments, the patient finally died of cardiac arrest and bradycardia. Infection with P. multocida was found in a patient with multiple sclerosis. Also, hospital-acquired infections with drug resistance caused by the weakness of the patient's system appeared in the patient who was hospitalized in the intensive care unit (ICU), and finally, the patient died. According to antibiotic patterns, the best antibiotic to which the bacteria is sensitive can be considered the primary treatment to avoid irrational antibiotic prescriptions.
Maryam Rezaeian, Laleh Hoveida,
Volume 19, Issue 1 (4-2025)
Abstract
Background: Infections caused by bacteria transmitted from food, including Enterobacteriaceae, Pseudomonas aeruginosa (P. aeruginosa), and Acinetobacter baumannii (A. baumannii) resistant to carbapenems, are spreading, and this has caused concerns in the field of treatment. This study investigated the frequency of carbapenem resistance genes in Enterobacteriaceae, P. aeruginosa, and A. baumannii isolated from raw chicken meat.
Method: In this cross-sectional study, 100 samples of raw chicken meat were collected from Isfahan. Bacterial infection was evaluated and confirmed using biomedical tests. Antibiotic sensitivity tests were performed using disc diffusion for Enterobacteriaceae, P. aeruginosa, and Acinetobacter baumannii isolates. The frequency of genes encoding resistance to carbapenems (OXA-181, OXA-48, VIM, NDM, IMP, and KPC) was determined through polymerase chain reaction (PCR) analysis.
Results: Out of 100 samples, 70 were positive for bacterial infection, of which 30 were infected with Escherichia coli (E. coli), 14 samples with Klebsiella pneumoniae (K. pneumoniae), 9 samples with Salmonella typhimurium (S. typhimurium), 11 samples with P. aeruginosa, and 6 samples were infected with A. baumannii. The highest amount of antibiotic resistance was estimated to be tetracycline, cotrimoxazole, gentamicin, trimethoprim, and streptomycin, and the lowest amount was azithromycin and rifampin. Among genes encoding resistance to carbapenem, NDM and OXA-48 genes were the most commonly expressed, with a frequency of 60% and 28.24%, respectively.
Conclusion: The study found significant bacterial contamination, especially for Enterobacteriaceae, with notable antibiotic resistance to tetracycline. Carbapenem resistance genes NDM and OXA-48 were prevalent, indicating the urgency of addressing antibiotic resistance.
