Jithu Jacob, Swapna C Senan, Ramani Bhai,
Volume 19, Issue 1 (4-2025)
Abstract
Aim: The global distribution of Klebsiella pneumoniae that produce carbapenemase has been gradually increasing.This present study aimed to investigate the molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates from various clinical samples. Materials and Methods: In this study, 401 bacteria of Klebsiella isolates were isolated from various clinical samples according to standard protocol. The twelve carbapenem-resistant genes of Klebsiella pneumoniae isolates were detected using multiplex polymerase chain reaction (PCR). Results: Multiplex polymerase chain reaction (PCR) for identifying Class A β-lactamases producers (KPC), Class B β-lactamases producers (NDM), and Class D β-lactamases producers (OXA-48) were done. It was noted that 10 isolates expressed KPC followed by one isolate expressed NDM and one isolate expressed OXA-48. Conclusion: In the present study conclude that CP-CRK is a major health problem in the coming years and hence it is necessary to take all adequate measures to identify the resistant strains. Continuous monitoring of these resistant mechanisms is required to establish the changes in the prevalence and sensitivity pattern of MDR Klebsiella isolates. Urgent infection control measures coupled with antibiotic stewardship and strengthening of the healthcare infrastructure are to be instituted in our setting to prevent the spread of these Carbapenem-resistant genes of Klebsiella pneumoniae (KPC). Larger multicenter studies are required to thoroughly assess risk variables and historical trends in order to comprehend the dynamics of spread and efficient management strategies.
