Showing 10 results for Glucose
Aa Jafari Nodoushan,
Volume 2, Issue 1 (4-2008)
Abstract
Abstract Background and objectives: Germ tube-producing ability of C.albicans in human serum is one of the most important virulent factors inducing transformation yeast to mycelia form. This phenomenon can be altered by some environmental and nutritional factors. The purpose of this study was to investigate the effect of temperature, pH and glucose concentrations in germ tube formation of C. albicans in an in vitro system. Material and Methods: The germ tube production test in human serum (with normal glucose titer) in different temperature, pH, and glucose concentrations was conducted invitro using standard strain of C. albicans (ATCC 10231). The average number of cells with germ tube after 2 hours and the rate of yeastmycelial transition were analyzed using one-way ANOVA test. Results: Maximum germ tube production rate is seen in temperature of 37°C, pH of 6.5 and glucose concentration of 30 mg/ml (P= 0.0001) and also germ tube is seen in earliest time in these conditions in invitro. Conclusion: It seems that these environmental and nutritional factors in human body particularly in diabetics can make this fungus to produce germ tube and invade to the different tissues. Key words: Candida Albicans, germ tube, in vitro, Temperature, pH, Glucose
Nazari, H, Habibeh Najar, H, Emadi, A, Abasi, Y, Salehiyan, A, Monem, M, Ghods, F, Mohammadi, A., Khaleghian, A,
Volume 5, Issue 2 (10-2011)
Abstract
Abstract Background and objectives: Glucose-6-phosphate dehydrogenase (G6PD) is an enzyme in the pentose phosphate pathway. G6PD deficiency (an X-linked recessive hereditary disease) is an inherited condition affecting approximately 3% of the people globally. This deficiency can cause hemolytic anemia and jaundice in neonates. The goal of this study is to detect the prevalence of G6PD deficiency in neonates referred to Semnan province screening lab Material and Methods: This cross sectional study, from 2007 to 2010, was conducted on the basis of country planned program and in line with neonatal screening tests. Blood samples were taken from the heels of 3-5 day neonates. Assessment of G6PD was done by rapid fluorescent spot test. The Prevalence of G6PD deficiency in boys and girls was compared by chi square (p<0.05(test. Results: Of 9353 newborns referred to semnan province screening Lab., 4820(51.53%) are males and 4533(48.47%) females. Three hundred (3.2%) of them suffer from G6PD deficiency. Of these, 263 (5.45%) are males and 37 (0.81%) females. The ratio of male to female is 7 to 1. Conclusion: The prevalence of G6PD deficiency is detected 3.2% in Semnan province and its frequency in boys is more than that of girls, which is expected. Key words: Glucose -6-phosphate dehydrogenase, Neonatal screening, Fauvism
M Alimohammadi, M Rostami, M Jorfi,
Volume 6, Issue 2 (10-2012)
Abstract
Gestational diabetes mellitus (GDM) is the most common metabolic disorder during pregnancy which is associated with 3 to 4 folds of increase in the risk of maternal and fetal morbidity, if it is not diagnosed early (1). Gestational diabetes occurs from 1% to 14%, and 90% of pregnancy-related diabetes has been related to gestational diabetes (2). Glucose challenge test (GCT) and glucose tolerance test (GTT) are recommended by American Diabetes Association (ADA) for screening and diagnosing of gestational diabetes (3).
In this cross-sectional study, the subjects were the pregnant women during 24-28 weeks of gestation referred to Khatam-o-allanbia laboratory of Arak city in 2009. First, we explained the aim of the project and then obtained the written consent. After being fast for at least 8-12 hours, five ml of their venous blood was drown. For GCT, the blood samples were obtained one hour after consumption of 50g of glucose powder. The Individuals whose GCT was equal or higher than 130 mg/dl were introduced for GTT in the future days.
After 8-12 hours of being fast, blood samples were taken to perform GTT. Then after eating 100 grams of glucose powder, their 1, 2 and 3 hour -venous blood samples were drown to perform glucose test, using glucose testing kit of Pars Azmun Company and Mindary BS-300 autoanalyser.
The average age of referred pregnant women is 27.37±10.24 year. Of 417, 403 (96.64%) have normal fasting plasma glucose (≤ 99 mg/dl) and 14 (3.36%) have abnormal fasting plasma glucose (≥ 100 mg/dl). When normal GCT is considered ≥140 mg/dl and ≥130 mg/dl, 357 (85.61%) and 308 (73.56%) have normal GCT and 60 (14.39%) and 109 (26.14%) have abnormal GCT, respectively. The Average of fasting plasma glucose (FPG) and GCT are 83.86 (73-243) and 118.32 (140-351) mg/dl, respectively. In 9 (64.29%) of women with abnormal FPG, the GCT test is also abnormal.
By performing GTT for the women whose GCT was equal or higher than 130 mg/dl, 24 (5.76%) were identified with gestational diabetes and introduced for treatment. If 140 mg/dl was considered as a cutoff point of GCT, four women (0.94%) would falsely be non-diabetic. Based on this study, the prevalence of GDM is 5.74% that is not so high, compared to the range of 4.8 to 7.4% of the country (4). A study conducted by Soheilykhah et al. on 1071 pregnant women with GCT of ≥ 130 mg /dl, 31.9% of the women had abnormal GCT. After doing GTT, 110 patients (10.2%) have been diagnosed with gestational diabetes (2) that is higher than the prevalence of other studies in Iran.
Due to results of this study and other studies, it seems that the GCT is not a reliable test for screening gestational diabetes. On the other hand, if GCT is going to be used, further studies are required to review the current Cutoff point of this test.
Key words: Glucose challenge test (GCT), Fasting plasma glucose (FPG), Gestational diabetes.
J Asadi, S Eshghinia, Fa Taleban, Gr Vaghari, A Esmaillzadeh,
Volume 7, Issue 4 (1-2014)
Abstract
Abstract
Background and Objective: Carbohydrates are considered as the major source of energy in physical activity. Studies show that consumption of carbohydrate foods before exercise can balance blood glucose and free fatty acids and increase athletes’ performance. In this study , we compared the effect of three kinds of foods with different glycemic indices on blood glucose (BG) and serum free fatty acids (FFA) in cycling ,male athletes.
Material and Methods: In this clinical trial, 21 members of national cycling team randomly allocated to three equal groups of glucose (low glycemic index) ، lentil (low glycemic index) and potato (high glycemic index). First, Fasting blood samples (5ml) were obtained to measure BG and FFA . Then the subjects were asked to eat their foods. After 45 mins of rest, they pedaled with maximal oxygen consumption VO2max) for two hours and again their blood samples were taken to compare with the levels of before interventions.
Results: Glucose consumption resulted in a significant decrease in FFA level after 2 hours of pedaling (P = 0.01) but no significant change in BG level. Plasma glucose was higher after eating lentil than that of potato (P<0.05), but it was not true for FFA level of both groups.
Conclusion: based on the results, the pre-exercise use of low glycemic index (lentil) compared to high glycemic index (potato) can better lead to increased blood glucose during exercise.
Keywords: Glycemic Index, Blood Glucose, Serum Free Fatty Acids, Cyclists
Mohammad Taghvaie, N, Jalali, Mt, Ghasemi Falavarjani, M, Shahbazian, Bb, Saki, A,
Volume 9, Issue 2 (7-2015)
Abstract
Background and Objective: According to recent changes in diagnostic criteria for diabetes, the harmonization of results obtained from various methods and systems by considering their accuracy and precision is essential. This study aimed to evaluate the accuracy, precision and consensus of some routine laboratory glucose kits in comparison with Hexokinase reference method.
Material and Methods: The participants were 38 diabetic patients with fasting blood sugar (FBS) ≥126 mg/dl, nine prediabetic patients with FBS of 100-125 mg/dl, 15 non-diabetic people with FBS of 60-100 mg/dl and 9 hypoglycemic patients with FBS of ≤60 mg/dl. Their FBS were measured by four routine laboratory glucose kits: Glucose oxidase on BT3000 analyzer with an open system and Hexokinase reference method on a close system (COBAS INTEGRA®400plus analyzer, Roche kit). Accuracy and precision were determined and compared with reference method.
Results: Glucose oxidase methods showed a good agreement with the reference method, in Correlation Coefficient>0.99. based on regression analysis, the slope of 1.114 for Pars Azmoon, 1.105 for Bionik, 1.121 for Elitech and 1.087 for Human were reported (P<0.05). Error of the mean for ParsAzmoon was 12.79, for Bionik 10.86, for Elitech 12.58 and for Human were 8.46. Coefficient of Variation showed more imprecision for Bionik and Human kits.
Conclusion: given the same almost standard errors, standard devisions and regression analysis, the precision in four methods is the same but in comparison with Hexokinase, reference method has not the accuracy.
Keywords: Blood Glucose, Glucose Oxidase, Hexokinase, Methods, Consensus
Maryam Moradibinabaj , Mohadese Namjoo , Mojgan Nejabat , Hamidreza Joshaghani ,
Volume 10, Issue 1 (1-2016)
Abstract
ABSTRACT
Background and Objective: The association of Triglyceride/High Density Lipoprotein-Cholesterol (TG/HDL-C) ratio with fasting serum insulin, which is an alternative method of insulin resistance (IR) measurement, is well-recognized. Thus, the measurement of TG/HDL-C ratio is useful to determine both IR and dyslipidemia, which itself is a characteristic of individuals with IR. Therefore, this study aimed to investigate the relationship between TG/HDL ratio as an indicator of IR, with different fasting blood glucose levels.
Methods: This case-control study was performed on 343 volunteers with no history of diabetes or use of blood glucose-lowering medications and fasting blood sugar (FBS) levels of less than 126 mg/dl. After sampling, the subjects were divided into three groups based on their FBS level. First group included healthy subjects with FBS of less than 100 mg/dl. Second group consisted of subjects with impaired fasting glucose (IFG) and FBS of 110-100 mg/dl and a third group including those with impaired glucose tolerance (IGT) and FBS of 110-125 mg/dl.
Results: The amount of TG/HDL-C ratio was 3.8 ± 2.8, 4.0 ± 2.1 and 5.4 ± 3.8 for the healthy group, individuals with IFG and IGT, respectively. The TG/HDL index was significantly different among the tested groups with no significant difference between healthy subjects and subjects with IFG. Moreover, there was a statistically significant difference between the IGT and IFG groups with healthy individuals.
Conclusion: Considering the significant increase of the TG/HDL ratio in groups with impaired glucose, using this index can be helpful in evaluation of glycemic disorder.
Mahtab Nejadaria, Seyed Mojtaba Mortazavi, Mohammad Hasan Kohansal,
Volume 14, Issue 2 (3-2020)
Abstract
ABSTRACT
Background and Objectives: The pentose phosphate pathway is of particular importance for energy supply in red blood cells. Glucose 6-phosphate dehydrogenase is the key enzyme involved in this pathway. The deficiency of this enzyme due to lack of nicotinamide adenine dinucleotide phosphate (NADPH) production in red blood cells leads to oxidation, hemoglobin deposition, red blood cell membrane changes and favism, which causes jaundice and hemolytic anemia in newborns. In this study, we evaluate the frequency of G6PD deficiency in newborns in a hospital in Bam, Iran.
Methods: In this descriptive-analytical cross-sectional study, blood samples were taken from 572 newborns hospitalized at Pasteur hospital of Bam (Iran) in the first half of 2018. Demographic data including gender and blood type were collected. The G6PD enzyme activity was evaluated using qualitative G6PD assay kit (Saba Teb, Iran). Data were analyzed using SPSS software.
Results: Of the 572 cases examined, 52 (9.09%) had G6PD deficiency. Of the affected patients, 34 (65.4%) were male and 18 (34.6%) were female. In addition, the majority (47.7%) of affected patients were with O+ blood group.
Conclusion: A relatively high frequency of G6PD deficient infants was reported in Bam. As expected, the prevalence of this disorder was higher in males than in females. Given the lost cost of screening, we suggest screening for G6PD enzyme activity in all newborns.
Keywords: Favism, Glucose 6-Phosphate Dehydrogenase, NADPH.
Marzeyeh Mirzazadeh Bakhteyari , Saied Shakerian, Mohsen Ghanbarzadeh,
Volume 15, Issue 3 (5-2021)
Abstract
Background and objectives: The prevalence of obesity and overweight in women is increasing. Secretion of adipokines such as vaspin may affect insulin sensitivity. It has been suggested that physical activity can alter circulating vaspin levels. The purpose of this study was to investigate effects of six weeks of aerobic interval training on serum vaspin and blood glucose levels in obese inactive girls.
Methods: In this quasi-experimental study, 22 inactive overweight girls were selected from the Shahid Chamran University of Ahvaz, Iran. The mean age, weight and body mass index of the subjects were 24.22±1.35 years, 83.77±12.66 kg and 32.56±3.36 kg/m2, respectively. The subjects were randomly divided into an experimental group )n=11) and a control group )n=11). The experimental group performed aerobic training on stationary bicycle at 65-80% of maximal strength, three sessions a week for six weeks. Serum levels of vaspin and glucose were measured using commercial kits. Data were analyzed using SPSS (version 23) and at significance of 0.05.
Results: The training intervention had no significant impact on the serum vaspin and glucose concentrations (P>0.05). In addition, the covariance analysis showed that serum vaspin and glucose levels did not differ significantly between the study groups (P>0.05).
Conclusion: Based on the results, the six-week aerobic interval training had no significant effect on vaspin and blood glucose levels of inactive obese girls. It is recommended to further examine the effect of this type of exercise at higher intensities and for longer durations.
Alireza Ahmadi, Mahdi Ghasemian , Ali Asghar Ayatollahi, Murtadha Al-Khabori, Seyedeh Somayeh Hosseini Alarzi, Mohammad Taher Hojjati,
Volume 15, Issue 5 (9-2021)
Abstract
Background and objectives: Diagnosis glucose intolerance in pregnancy is very important in preventing maternal and fetal complications. In this study, we compared hematological and biochemical characteristics of healthy pregnant women and women with gestational diabetes mellitus (GDM) to find predisposing and prognostic variables of GDM.
Methods: In this study, 80 pregnant women (at 24-28 weeks of pregnancy) were divided into a GDM group and non-GDM group by performing oral glucose tolerance test using 75 g glucose according to the International Association of the Diabetes and Pregnancy Study Groups criteria.
Results: The mean age of women with GDM was significantly higher than those without GDM (p=0.048). Other variables including body mass index, gestational age and daily sleep duration did not differ significantly between the two groups (P>0.05). There was a significant association between family history of diabetes and incidence of GDM (p=0.040). In addition, the C-peptide level was significantly higher in pregnant women with GDM (p=0.004).
Conclusion: Considering the role of C-peptide in predicting metabolic syndrome, it is suggested to use this factor for identification of GDM patients.
Parisa Hasanein, Fahime Javadi Hedaiat Abad, Musa Bohlooli, Mostafa Khajeh, Sedigheh Esmaielzadeh Bahabadi, Neda Poormolaei,
Volume 19, Issue 2 (3-2025)
Abstract
Introduction: DNA Glycation damages DNA by inducing breaks of strands, mutations, and finally changes in gene expression, which is assumed as a main factor in pathogenesis of diabetes and its complications. Therefore, antiglycation agents have been focused recently for preventing and alleviating diabetes complications. According to the reported antidiabetic effects of Tamarix aphylla (T. aphylla) leaves extract, this study was aimed to determine the effect of T. aphylla on glucose-mediated DNA glycation for the first time.
Methods: DNA incubated with glucose for 4 weeks and the inhibitory or fascilitatory effects of T. aphylla on DNA structural changes were studied by various techniques. These techniques were included UV–Vis, fluorescence spectroscopy, circular dichroism (CD) and agarose gel electrophoresis.
Results: The findings of UV–Vis and fluorescence spectroscopy showed that T. aphylla decreased the DNA-AGE (advanced glycation end products) formation. Based on the CD and agarose gel electrophoresis results, the structural changes of glycated DNA was decreased in the presence of T. aphylla.
Conclusion: Thus T. aphylla has beneficial effects against DNA glycation and could be a promising agent for ameliorate the adverse effects of glycation in the presence of glucose and conditions of raised blood glucose after confirming in further studies.
