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Showing 3 results for Inflammation
The evaluation of the effect of periodic resistance training along with vitamin D3 consumption and mesenchymal stem cell transplantation on cortical TNF-β levels in streptozotocin-induced diabetic rats
Behrooz Mohammadnezhad, Seyed Abdollah Hashemvarzi ,
Volume 19, Issue 3 (5-2025)
Abstract
Background: Inflammation plays a major role in the development and progression of diabetes. Vitamin D deficiency and physical inactivity can also increase the risk of developing type 2 diabetes. Combined therapeutic strategies are promising approaches for the treatment and prevention of diabetes. This study aimed to investigate the effects of resistance training, vitamin D3 supplementation, and adipose-derived mesenchymal stem cell (MSC) transplantation on tumor necrosis factor-beta levels in the cerebral cortex of diabetic rats.
Methods: Eighty male Wistar rats (Weighing 290±19 g) were randomly divided into 10 groups: healthy control, sham, diabetes, training, vitamin D, MSC, training+vitamin D, MSC+training, MSC+vitamin D, and training+MSC+vitamin D. Training groups were subjected to a resistance training program on a ladder. MSC groups received 1.5 × 106 MSCs, and vitamin D supplementation groups received 1 microgram/kilogram of vitamin D3 eight times. Cortical TNF-β levels and fasting serum glucose levels were measured.
Results: After six weeks, the combination of resistance training with vitamin D3 supplementation and MSC transplantation (P=0.018), as well as the combination of resistance training with MSCs (P=0.024), significantly reduced the diabetes-induced elevation of TNF-β levels.
Conclusion: Resistance training with appropriate intensity, duration, and recovery between exercise sessions, combined with MSC transplantation and vitamin D3 supplementation, has profound anti-inflammatory effects on the cerebral cortex tissue of diabetic rats. This type of intervention, especially the transplantation of MSCs, may be a promising protective strategy against some complications of diabetes.
 

Effect of a period of intense functional training on IL-10, Dectin-1, and IL-1Ra in prediabetic obese women
Maryam Enshaei Mojarad , Hajar Abbaszadeh , Parvin Farzanegi ,
Volume 19, Issue 4 (7-2025)
Abstract
Background: Obesity and prediabetes are associated with chronic low-grade inflammation, and macrophage-related markers such as IL-10, Dectin-1, and IL-1Ra play a key role in modulating inflammatory responses. The purpose of this study was to investigate the effect of a period of high-intensity functional training (HIFT) on IL-10, Dectin-1, and IL-1Ra in prediabetic obese women to assess the impact of this exercise modality on M2 macrophage markers.
Methods: Thirty eligible female volunteers aged 35-40 years were selected and homogeneously divided into two groups: 1) control (n=15) and 2) training (n=15). The training group underwent a 16-week HIFT program based on CrossFit protocols, incorporating squats, deadlifts, barbell/dumbbell exercises, kettlebell swings, and aerobic/weight-bearing movements in a Workout of the Day format (60 min/session). The control group maintained their daily routines without structured exercise. Serum levels of Dectin-1, IL-10, and IL-1Ra were measured via ELISA. Descriptive statistics (Mean, standard deviation) were used for data analysis.
Results: After 16 weeks, HIFT significantly reduced Dectin-1 (P = 0.048) and increased IL-10 (P < 0.0001) and IL-1Ra (P < 0.0001) levels in prediabetic obese women.
Conclusion: These findings suggest that 16 weeks of HIFT may enhance anti-inflammatory markers (IL-10, IL-1Ra) and modulate Dectin-1, potentially mitigating obesity-related inflammatory complications in prediabetic women.
 

Effect of interferon- on the expression of regulatory NLRP6 and NLRP12 genes in human Wharton’s jelly mesenchymal stem cells
Leila Pirdel, Maryam Safajoo, Masoud Maleki,
Volume 19, Issue 5 (9-2025)
Abstract
Background: Mesenchymal stem cells (MSCs) are well-known for their immune-modulatory properties. A subgroup of the nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) has been recently identified to play a regulatory role in immune and inflammatory responses. This study aims to analyze and compare the gene expression levels of the NOD-like receptor family pyrin domain-containing proteins (NLRPs), such as NLRP6 and NLRP12, in Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) treated with interferon-gamma (IFN-γ), the pro-inflammatory cytokine, and untreated cells.
Methods: The immunophenotypic characterization of the isolated Wharton’s Jelly mesenchymal stem cells (WJ-MSCs) was conducted using flow cytometry. Next, they were cultured with or without IFN-γ, followed by a comparison of the expression levels of the NLRP6 and NLRP12 genes using quantitative PCR (qPCR).
Results: Treatment of cells with IFN-γ resulted in a statistically significant increase in NLRP12 gene expression compared to untreated cells. In contrast, the expression of NLRP6 did not differ significantly between cells with or without IFN-γ treatment.
Conclusion: The altered expression level of NLRP12 suggests its potential role in the inflammatory regulation mediated by WJ-MSCs in response to IFN exposure; however, further studies are needed to validate its role in experimental models of inflammatory-related diseases.

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