Showing 4 results for Microorganism
Hr Tavakoli, M Manafi, M Bayat, A Mehrabi Tavana,
Volume 2, Issue 2 (10-2008)
Abstract
Abstract Background and Objectives: Chromogenic media are the newest methods applied for rapid detection of pathogenic microorganisms in drinking water and food from 1998-2008. These Specific media contained the compounds acting as a substrate for microbial enzymes and, according to the type of enzyme, produce specific color. The aim of this study was to introduce the chromogenic media as a powerful tool in rapid detection of pathogenic agents in drinking water and food. Material and Methods: In this review article, the published papers about the use of chromogenic media in rapid detection of water and food-born pathogens were investigated. Results: The studies conducted in different countries show that the chromogenic media are very sensitive, specific and with high performance therefore, we can use it to detect the most important pathogenic microorganisms (such as Salmonella spp, E.coli, S.aureus, L.monocytogenes, and Candida spp.) in water and food samples. Conclusion: Because chromogenic media, in comparison with the other rapid detection methods such as PCR and ELISA, are very sensitive and cheaper, it can be applied as an alternative method. Key words: Chromogenic media, Rapid detection, water and food, Microorganism
F Sedighian, A Sanee, H Alaouddoulee, M Arshi, Kh Rekabpoor,
Volume 2, Issue 2 (10-2008)
Abstract
Abstract Background and objectives: Resistance to antimicrobial agent is a world-wide problem and the existed reports are not the same. Aware of antibiotic resistant pattern of microorganisms in each hospital is important for controlling the nosocomial infections. This study was designed to determine the resistant pattern of microorganisms isolated in Yahya nejad hospital, Babol, north of Iran. Material and Methods: In this descriptive study, organisms were isolated during 2006 from urine, blood, endotracheal tube, abscess, fluids (ascites, pleura, and synovia), wound, discharges and bonemarrow of both outpatients and inpatients in our hospital were studied. Their antibiotics resistances were evaluated by Kirby Bauer method. Results: of 3114 culture specimens of admitted patients (female 1732, male 1382), 325 (10.4%) microorganisms were isolated. They were isolated from urine 162(49.8%), blood 115(35.4%), abscess 11(3.4%), wound, 10(3.1%) and discharge10 (3.1%). The most common microorganisms were Escherichia coli 107(33%) and Staphylococcus epidermidis 69(21.3%). Maximum resistance of E.coli is related to tetracycline (83.9%) and ampicillin (75%) and maximum resistance of S.epidermidis to oxacillin (89.2%) and penicillin (82.1%). Conclusion: With regard to high antibiotic resistance (>70%) in this study, it seems that the early beginning and inappropriate dosage of antimicrobial agents may be associated with developing antibiotic resistance. Hence, it is highly recommended to prohibit the unnecessary prescription of antibiotics Key words: Antibiotic resistance, microorganisms, Blood culture, E.coli
N Tayybeimeibodi, M Naderi Nasab, Y Nahide, A Javadi, M Afzal Aghaei,
Volume 4, Issue 2 (10-2010)
Abstract
Abstract Background and objectives: Pathogens can be transferred via the hands of the personnel not only to themselves but also to their families or to the patient causing infection especially nosocomial infections. Microbial contamination of hand is caused by contact with patients and their environments or usual devices in the workplace. It seems that contamination of computer devices and handsets are more in hospital than official buildings. The aim of this study was to assess and compare the microbial contamination of computer keyboards, mouse and telephone receivers in a hospital department and an official building. Material and Methods: the sterile swab samples obtained from 32 keyboards, 31 computer mouse and 30 telephone receivers in the official building of Mashhad medical university and central laboratory of Imam Reza hospital were cultured on Blood agar and MacConkey agar plates. Results: Out of 64 samples from the official building, we identify 83 microbial germs. The most common ones are gram-positive Bacilli (n =34, 40.95%) and coagulase-negative Staphylococci (n = 32, 32.53%). Of 29 samples of central laboratory, there are 33 microbial germs .The most common of them are grampositive Bacilli (n = 19, 57.57%) and coagulase-negative Staphylococci (n = 7, 21.21%). Overall, microbial contamination of the computer equipment and handsets is not statistically meaningful (P< 0.05). Some germs like diphtheroid are not existed in laboratory, but two cases of Aspergillus are found. Conclusion: The presence of most of the germs on these devices is due to dusting or normal flora transferred via staffs’ hands. Only two of them, coagulase-positive Staphylococci and Aspergillus, should be considered carefully because they may cause serious infections in staff, their families or patients. Key words: contamination , workers’ desk, microorganism, computer keyboard and mouse
Khandan Del, A, Ghaemi, Ea,
Volume 9, Issue 3 (9-2015)
Abstract
Background and Objective: Bacterial infections are of the leading causes of morbidity and mortality in ICU patients with underlying immunodeficiency. This study aimed to determine the frequency of microorganisms in patients admitted to the ICU of Panje Azar Hospital in Gorgan.
Material and Methods: This cross-sectional study was conducted on the patients admitted in three wards, each having at least 48, of ICU in Panje Azar Hospital in Gorgan. All microbial cultures requested by patients' physician were recorded.
Results: In ICU positive cultures were 53.9%. The number was 16 for Staphylococcus coagulase negative, 15 for Enterococci , 11 for Yeast, 11 for E.Coli, 9 for Enterobacter , 8 for streptococcus, 5 for staphylococcus aureus. The highest percentage was related to coagulase-negative staphylococci (16.5%) and the lowest to Alcaligenes, Diphtheroids, Acremo bacteria , Serratia with one positive case.
Conclusions: the incidence of bacterial contamination in ICU patients compared to other parts of country is not different significant. While it is far from world standard, it is essential that many efforts be done to reduce the level of infection.
Keywords: ICU; Microorganisms; Panje Azar Hospital.
