Sun, Jun 29, 2025
1- VARDHMAN MAHAVIR MEDICAL COLLEGE, NEW DELHI
2- VARDHMAN MAHAVIR MEDICAL COLLEGE, NEW DELHI ,anjalisharmapath@gmail.com
3- Department of Pathology, Vardhman Mahavir Medical College & Safdarjung Hospital, New Delhi, India.
2- VARDHMAN MAHAVIR MEDICAL COLLEGE, NEW DELHI ,
3- Department of Pathology, Vardhman Mahavir Medical College & Safdarjung Hospital, New Delhi, India.
Abstract: (67 Views)
Background & objectives: Sepsis is one of the most common cause of mortality among patients who are critically ill and in Intensive Care Units(ICU). Bacterial infection or sepsis leads to an increase in Neutrophilic CD64(nCD64) expression on activated polymorphonuclear leukocytes(PMNs). Early diagnosing of sepsis is very important in order to start timely and specific treatment. The availability of a rapid laboratory test with high specificity for sepsis in adult patients could aid in therapeutic decision making and reduce unnecessary antibiotic use.
Methods: Sepsis patients diagnosed as per sepsis-3 definition were included in this study. 2 ml blood sample was collected in EDTA & plain vial each for evaluation of nCD64, Procalcitonin(PCT) & high sensitivity C-reactive protein(hS-CRP). The samples were run on Flow cytometer, Nephelometer and Chemiluminescence for nCD64, hS-CRP and PCT respectively.
Result: The Positive Predictive Value(PPV) of nCD64 for prediction of sepsis was 92.68% and the Negative Predictive value(NPV) was 94.87%. Receiver operating curve(ROC) was plotted for indicating the diagnostic accuracy of nCD64(≥1.8), hS-CRP(≥3 mg/L) and PCT(≥0.4 ng/mL). Area under the curve(AUC) for nCD64 was highest[0.938(95%CI=0.876-0.999)] followed by hS-CRP[0.888(95%CI=0.807-0.968)] and PCT [0.850(95%CI =0.759-0.941)].
Conclusion: These findings are suggestive of the possibility that nCD64 determination was a useful tool for diagnosing infection in patients with septic syndrome, with a performance superior to that of hS-CRP and PCT.
Methods: Sepsis patients diagnosed as per sepsis-3 definition were included in this study. 2 ml blood sample was collected in EDTA & plain vial each for evaluation of nCD64, Procalcitonin(PCT) & high sensitivity C-reactive protein(hS-CRP). The samples were run on Flow cytometer, Nephelometer and Chemiluminescence for nCD64, hS-CRP and PCT respectively.
Result: The Positive Predictive Value(PPV) of nCD64 for prediction of sepsis was 92.68% and the Negative Predictive value(NPV) was 94.87%. Receiver operating curve(ROC) was plotted for indicating the diagnostic accuracy of nCD64(≥1.8), hS-CRP(≥3 mg/L) and PCT(≥0.4 ng/mL). Area under the curve(AUC) for nCD64 was highest[0.938(95%CI=0.876-0.999)] followed by hS-CRP[0.888(95%CI=0.807-0.968)] and PCT [0.850(95%CI =0.759-0.941)].
Conclusion: These findings are suggestive of the possibility that nCD64 determination was a useful tool for diagnosing infection in patients with septic syndrome, with a performance superior to that of hS-CRP and PCT.
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.